Global spectra of energy and enstrophy and their fluxes during July 1979

Transient and stationary spectra of kinetic energy (KE), available potential energy (APE) and enstrophy (EN), and their spectral fluxes as a function of the two-dimensional wavenumbern were computed for July 1979. Triangular truncation at zonal wavenumber 42 was used for computation. The slopes of various spectra in the wavenumber range 14≤n≤25 were obtained by fitting a straight line in log-log scale by the least square method. The transientKE, APE andEN spectra in the lower (upper) troposphere had slopes −2·21 (−2·30), −2·65 (−2·64) and −0·36 (−0·46), respectively. The effect of stationary and divergent motion on the slope values was investigated. The possible correlation between the slope and percentage of transient component in the combined energy and enstrophy was examined to identify the transient motion of the atmosphere with the two-dimensional homogeneous isotropic turbulence. The vertically averaged slope of kinetic energy and enstrophy in the lower (upper) troposphere was close to the value at 700 (200) hPa leve

Fresnel zone plate telescopes for X-ray imaging II: numerical simulations with parallel and diverging beams

We present the results of simulations of shadows cast by a zone plate telescope which may have one to four pairs of zone plates. From the shadows we reconstruct the images under various circumstances. We discuss physical basis of the resolution of the telescope and demonstrate this by our simulations. We allow the source to be at a finite distance (diverging beam) as well as at an infinite distance (parallel beam) and show that the resolution is worsened when the source is nearby. By reconstructing the zone plates in a way that both the zone plates subtend the same solid angles at the source, we obtain back high resolution even for sources at a finite distance. We present simulated results for the observation of the galactic center and show that the sources of varying intensities may be reconstructed with accuracy. Results of these simulations would be of immense use in interpreting the X-ray images from recently launched CORONAS-PHOTON satellite.Comment: 17 pages, 36 figures, Published in Experimental Astronom

On-farm evaluation of integrated nutrient and pest management in Cicer arietinum L.

Chickpea (Cicer arietinum L.) is the world’s third most important pulse crop. In chickpea, wilt caused by Fusarium oxysporum f. sp. ciceri is one of the major production constraints. On-farm demonstration on use of Trichoderma viride, PSB and Rhizobium was conducted during rabi (Nov-Jan) 2006 in Machanapally village, Ranga Reddy district, AP, India. At harvest, data on wilt incidence yield and related parameters were recorded. In T1, where seeds were treated with PSB+Rhizobium+T. viride followed by soil application of T. viride+PSB+Rhizobium after 30 DAS (mixed with 200 kg of FYM), wilt incidence was 4.5% and yield was 0.995 t.ha-1. In T2, where carbendazim was applied as seed treatment followed by the soil application of recommended doses of NPK, wilt incidence was 8.3% with a yield of 1.2 t.ha-1. In T3, where seeds were treated as in T1 and NPK was applied to soil as in T2, wilt incidence was 3.3% and crop yield was 1.13 t. ha-1. Highest plant dry mass (25.5 g.plant-1) and number of effective nodules (6.3 plant-1) were recorded in T3. To conclude, integrated nutrient and disease management is an ideal approach for chickpea cultivation

Design principles for riboswitch function

Scientific and technological advances that enable the tuning of integrated regulatory components to match network and system requirements are critical to reliably control the function of biological systems. RNA provides a promising building block for the construction of tunable regulatory components based on its rich regulatory capacity and our current understanding of the sequence–function relationship. One prominent example of RNA-based regulatory components is riboswitches, genetic elements that mediate ligand control of gene expression through diverse regulatory mechanisms. While characterization of natural and synthetic riboswitches has revealed that riboswitch function can be modulated through sequence alteration, no quantitative frameworks exist to investigate or guide riboswitch tuning. Here, we combined mathematical modeling and experimental approaches to investigate the relationship between riboswitch function and performance. Model results demonstrated that the competition between reversible and irreversible rate constants dictates performance for different regulatory mechanisms. We also found that practical system restrictions, such as an upper limit on ligand concentration, can significantly alter the requirements for riboswitch performance, necessitating alternative tuning strategies. Previous experimental data for natural and synthetic riboswitches as well as experiments conducted in this work support model predictions. From our results, we developed a set of general design principles for synthetic riboswitches. Our results also provide a foundation from which to investigate how natural riboswitches are tuned to meet systems-level regulatory demands

Conceptualisation, Development, Fabrication and In Vivo Validation of a Novel Disintegration Tester for Orally Disintegrating Tablets

Disintegration time is the key critical quality attribute for a tablet classed as an Orally Disintegrating Tablet (ODT). The currently accepted in vitro testing regimen for ODTs is the standard United States Pharmacopeia (USP) test for disintegration of immediate release tablets, which requires a large volume along with repeated submergence of the dosage form within the disintegration medium. The aim of this study was to develop an in vivo relevant ODT disintegration test that mimicked the environment of the oral cavity, including lower volume of disintegration medium, with relevant temperature and humidity that represent the conditions of the mouth. The results showed that the newly developed Aston test was able to differentiate between different ODTs with small disintegration time windows, as well as between immediate release tablets and ODTs. The Aston test provided higher correlations between ODT properties and disintegration time compared to the USP test method and most significantly, resulted in a linear in vitro/in vivo correlation (IVIVC) (R 2 value of 0.98) compared with a "hockey stick" profile of the USP test. This study therefore concluded that the newly developed Aston test is an accurate, repeatable, relevant and robust test method for assessing ODT disintegration time which will provide the pharmaceutical industry and regulatory authorities across the world with a pragmatic ODT testing regime

Acute Drug Treatment in the Early C. elegans Embryo

Genetic and genome-wide RNAi approaches available in C. elegans, combined with tools for visualizing subcellular events with high-resolution, have led to increasing adoption of the early C. elegans embryo as a model for mechanistic and functional genomic analysis of cellular processes. However, a limitation of this system has been the impermeability of the embryo eggshell, which has prevented the routine use of small molecule inhibitors. Here, we present a method to permeabilize and immobilize embryos for acute inhibitor treatment in conjunction with live imaging. To identify a means to permeabilize the eggshell, we used a dye uptake assay to screen a set of 310 candidate genes defined by a combination of bioinformatic criteria. This screen identified 20 genes whose inhibition resulted in >75% eggshell permeability, and 3 that permeabilized embryos with minimal deleterious effects on embryo production and early embryonic development. To mount permeabilized embryos for acute drug addition in conjunction with live imaging, we combined optimized inhibition of one of these genes with the use of a microfabricated chamber that we designed. We demonstrate that these two developments enable the temporally controlled introduction of inhibitors for mechanistic studies. This method should also open new avenues of investigation by allowing profiling and specificity-testing of inhibitors through comparison with genome-wide phenotypic datasets

Systemic and Mucosal Immune Responses to Sublingual or Intramuscular Human Papilloma Virus Antigens in Healthy Female Volunteers

The sublingual route has been proposed as a needle-free option to induce systemic and mucosal immune protection against viral infections. In a translational study of systemic and mucosal humoral immune responses to sublingual or systemically administered viral antigens, eighteen healthy female volunteers aged 19–31 years received three immunizations with a quadravalent Human Papilloma Virus vaccine at 0, 4 and 16 weeks as sublingual drops (SL, n = 12) or intramuscular injection (IM, n = 6). IM antigen delivery induced or boosted HPV-specific serum IgG and pseudovirus-neutralizing antibodies, HPV-specific cervical and vaginal IgG, and elicited circulating IgG and IgA antibody secreting cells. SL antigens induced ∼38-fold lower serum and ∼2-fold lower cervical/vaginal IgG than IM delivery, and induced or boosted serum virus neutralizing antibody in only 3/12 subjects. Neither route reproducibly induced HPV-specific mucosal IgA. Alternative delivery systems and adjuvants will be required to enhance and evaluate immune responses following sublingual immunization in humans

Measurement of the top quark mass using the matrix element technique in dilepton final states

We present a measurement of the top quark mass in pp¯ collisions at a center-of-mass energy of 1.96 TeV at the Fermilab Tevatron collider. The data were collected by the D0 experiment corresponding to an integrated luminosity of 9.7  fb−1. The matrix element technique is applied to tt¯ events in the final state containing leptons (electrons or muons) with high transverse momenta and at least two jets. The calibration of the jet energy scale determined in the lepton+jets final state of tt¯ decays is applied to jet energies. This correction provides a substantial reduction in systematic uncertainties. We obtain a top quark mass of mt=173.93±1.84  GeV

Ratio of the Isolated Photon Cross Sections at \sqrt{s} = 630 and 1800 GeV

The inclusive cross section for production of isolated photons has been measured in \pbarp collisions at $\sqrt{s} = 630$ GeV with the \D0 detector at the Fermilab Tevatron Collider. The photons span a transverse energy ($E_T$) range from 7-49 GeV and have pseudorapidity $|\eta| < 2.5$. This measurement is combined with to previous \D0 result at $\sqrt{s} = 1800$ GeV to form a ratio of the cross sections. Comparison of next-to-leading order QCD with the measured cross section at 630 GeV and ratio of cross sections show satisfactory agreement in most of the $E_T$ range.Comment: 7 pages. Published in Phys. Rev. Lett. 87, 251805, (2001